Contributions
Abstract: S482
Type: Oral Presentation
Presentation during EHA22: On Saturday, June 24, 2017 from 16:15 - 16:30
Location: Room N101
Background
Aims
In this study, we first aimed to validate Bcr-Abl mediated FcγRIIb upregulation on mRNA and protein level in leukemic cells. Next, we tested the effect of shRNA mediated FcγRIIb knock-down and depletion on CFU (colony forming unit) capacity, proliferation and leukemic signaling in vitro. Finally, we studied the disease-initiating potential of primitive CML stem and progenitor cells upon FcγRIIb knock down.
Methods
qRT-PCR and western blot analyses were applied using cell lines, primary murine cells and HoxB8 immortalized murine bone marrow (BM) cells for studying FcγRIIb expression and signaling. In order to test the biology of CML cells in vitro, we performed CFU and proliferation assays. Moreover, we performed viral infection of 5-FU treated SCLtTA/Bcr-Abl BM using FcγRIIb:shRNA or scrambled control and subsequent transplantation, followed by analyses of the disease, including immune-phenotyping, RNA and protein expression as well as histological analysis.
Results
Conclusion
Session topic: 7. Chronic myeloid leukemia - Biology
Keyword(s): Mouse model, Leukemic Stem Cell, Chronic myeloid leukemia
Abstract: S482
Type: Oral Presentation
Presentation during EHA22: On Saturday, June 24, 2017 from 16:15 - 16:30
Location: Room N101
Background
Aims
In this study, we first aimed to validate Bcr-Abl mediated FcγRIIb upregulation on mRNA and protein level in leukemic cells. Next, we tested the effect of shRNA mediated FcγRIIb knock-down and depletion on CFU (colony forming unit) capacity, proliferation and leukemic signaling in vitro. Finally, we studied the disease-initiating potential of primitive CML stem and progenitor cells upon FcγRIIb knock down.
Methods
qRT-PCR and western blot analyses were applied using cell lines, primary murine cells and HoxB8 immortalized murine bone marrow (BM) cells for studying FcγRIIb expression and signaling. In order to test the biology of CML cells in vitro, we performed CFU and proliferation assays. Moreover, we performed viral infection of 5-FU treated SCLtTA/Bcr-Abl BM using FcγRIIb:shRNA or scrambled control and subsequent transplantation, followed by analyses of the disease, including immune-phenotyping, RNA and protein expression as well as histological analysis.
Results
Conclusion
Session topic: 7. Chronic myeloid leukemia - Biology
Keyword(s): Mouse model, Leukemic Stem Cell, Chronic myeloid leukemia