Transient inhibition of NF-κB signaling enhances ex vivo propagation of human hematopoietic stem cells
EHA Learning Center. Talkhoncheh M. Sep 1, 2018; 234224
Topic: 4Bc Mobilization, collection and manipulation of hematopoeitic stem cells
Mehrnaz Safaee Talkhoncheh
Mehrnaz Safaee Talkhoncheh
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Co-Authors: Agatheeswaran Subramaniam, Mattias Magnusson, Praveen Kumar, Jonas Larsson, Aurélie Baudet

Abstract: Despite extensive studies, defining culture conditions in which hematopoietic stem cells can be expanded ex vivo has been challenging. Here we show that chemical inhibition of the NF-κB signaling pathway leads to a significant improvement of hematopoietic stem cell function from ex vivo cultured human umbilical cord blood derived CD34+ cells. We found a distinct peak of activation of the NF-κB pathway shortly after cells were put in culture, and consequently inhibition of the pathway was both necessary and sufficient during the first 24 hours of culture where it reduced the levels of several pro-inflammatory cytokines. Taken together, NF-κB pathway inhibition facilitates propagation of hematopoietic stem cells in culture and may complement other strategies for hematopoietic stem cell expansion by relieving stress signals that are induced as an immediate response to culture initiation.

Article Number: 1444

Doi: 10.3324/haematol.2018.188466

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